Other: Near the beginning of the movie, Milla Jovovich reads a note that has been left on the desk. She didn't write it, as they demonstrate in the film. However, when they do show the person who wrote the note composing it, the handwriting is different from that in the note from the earlier scene.

Continuity: When Matt gets scratched by the mutated Licker at the end of the film, the scratches on his arm appear before the creature's claws actually hit him.

Revealing: The scene where the group is being attacked in dining hall B, they are trying to get the doors open. J.D. is firing at two zombies and turns away to open the doors himself because Kaplan keeps forgetting the code. If you look closely, one of the zombies reacts a second late from being shot down.

Factual error: When the special forces arrive in the Station of the hive, the computer identifies the soldier`s weapons. The name under the picture is right, but the picture always shows a MP5, even at the soldier carrying a G36 Assault rifle.

Continuity: When JD asks why the zombie still was standing after he fired five shots at it, Rain has hair hanging down on the left side of her face. When it cuts, hair hangs down on both sides.

Continuity: In the first scene, the man in the suit wears a surgical mask over the lower half of his face. When Spence remembers it later, he wears no mask under the suit.

Factual error: In the laser corridor, when the grid of lasers is formed, some of the lasers rotate to form the grid, and are no longer emitted from anything, they are just hanging in the air.

Continuity: When the zombie dog jumps through the window Alice makes a run through the metal door and you can see the door is clean. When it cuts there is lots of blood on the door even before the zombie dog jumps on it.

Revealing: On the DVD commentary, the director says that he only had a handful of the experiment cubes in Dining Hall B and used cardboard cutouts for all the others. This is most obvious when Rain is walking through the hall alone to check on the first zombie: the cubes in the distance don't match the rigid rows of the ones in the foreground.

Plot hole: Red Queen is extremely conveniently programmed for the plot of the movie. 1. Rather than seal off rooms in a place she has TOTAL control of, (that by the way is designed to be isolated in sections for this very reason), she lets the infection spread to everybody in the Hive. She could have easily contained it in a small section of the facility. This is standard procedure in ANY biowarfare lab, there is no way she wouldn't be programmed to do it. 2. Rather than let everyone know the virus is loose, she just starts killing everyone. She had counter-virus available, why not let everyone know that they are locked in with the virus, but help is coming? The infected personnel would be hit with it only minutes after infection. If it didn't work and they died, they are still locked in. 3. Instead of calling Umbrella and telling them the virus is loose, she clams up and just slaughters everyone. This guarantees that Umbrella will ignore anything she has to say, and breach the Hive with a response team and release the virus, the very thing she is programmed to prevent. 4. When Kaplan is disabling her, she keeps telling him that it's a bad idea, but doesn't bother to let him know that shutting her down will release hordes of flesh-eating killers. 5. She just assumes that Spence, no matter how he got in to the T-virus storage has clearance to stuff it all in a duffel bag and walk out with it. She could have locked him in and double-checked with Umbrella. 6. The entire reason for her existence is to prevent the virus from escaping, while almost every decision she makes greatly increases the chances it will get out of the Hive. This is way beyond bad programming - this is highly advanced AI doing the exact opposite of what she's designed for.

Deliberate "mistake": When the woman is attempting to escape from the elevator, it dislodges and falls down, so then the actress is facing the ground...but when her arm hits the floor, the floor itself bends. If you watch the DVD with the commentary on, the director points out that it was a break away floor in case the actress' head accidentally snapped forward, to avoid any injury.

Continuity: When Alice awakens in what looks like a stainless steel bin, there is a long scratch on her right shoulder. Throughout the rest of the movie, the scratch is seen on her left shoulder.

Plot hole: Why does Umbrella know that Red Queen killed everyone, but not that the virus is loose? Is there no way to see into or receive data from the Hive other than Red Queen? Does every communication in or out have to be approved through her? And even if she is the sole conduit for information into and out of the Hive, her primary purpose for existing is to keep the T-virus from escaping. Umbrella THINKS that Red Queen has malfunctioned. BUT, she is working perfectly! So supressing info that the T-virus is loose in the Hive would contradict her core programming. So why doesn't Umbrella know?

Audio problem: When the code is entered for the door after the first zombie fight, the guy entering it says "got it". His lips aren't in sync with the words.

Other: When the train arrives back under the mansion from the Hive, it enters the station from the wrong direction. Looking at the track from the platform, the train comes in from right to left, when it should be from left to right.

Continuity: When Alice escapes the first zombie dog and locks herself in the room, a zombie approaches her and she kicks him down. When the zombie falls you can see his tie is partially outside of his suit. When it cuts to Alice reaching for his gun, the tie is tucked inside his suit.

Continuity: Shortly after the Licker pulls Kaplan out of the train, Matt shuts the door so it can't get at the rest of them. The Licker goes on the roof to use the back entrance to get into the car. Matt hears this and rushes to bar the back door. Pay attention to the near dead Raine. She suddenly gets a lot more life and quickly moves out of the way to avoid being accidently trampled.

Other: Watch Milla Jojovich's hair during the scene when they are held at gunpoint by Spence. Her hair moves from shot to shot. For the most obvious, watch right after Spence shoots the zombie.

Continuity: When the first laser shoots through the room, the woman soldier is looking at it with the others; yet when the shot cuts to her just before she loses her head, she only just turns in time to see it before it reaches her.

Audio problem: In one of the scenes right before the solider get killed by the lasers and the alarm is sounding, the commander says to everyone "Hold your positions, everyone stay calm" but his lips aren't moving.