Deliberate "mistake": During the scene where Carlos and L.J. are searching the motel, L.J. sees a zombie which turns out to be a reflection in a wall-length mirror. L.J. shoots, breaking the mirror and realizing his mistake. But, if he had been standing in front of the mirror as shown, he should have seen his own reflection standing there as well. This was done as an homage to a similar encounter in the original game.

Revealing: Near the end of the movie when they are showing Tokyo and the camera pans down through the street to Umbrella's underground HQ, you can see a rat leaning over the 'edge' of the road as it is going down.

Revealing: As the mutated zombies chase some of the survivors up the cement ramp in Las Vegas, watch the very last zombie just behind the girl in the tank top. He slows himself down to a fast walk when the actress starts running too slow.

Continuity: After the Zombies are unleashed in the desert scene, we see Carlos crawling under the truck. At first his semi-automatic gun seems to be completely normal. The gun's slide and chamber are closed as if there are still bullets in the magazine or at least one bullet is chambered. But then the camera focuses on his gun and we see the gun's chamber is open - as if there are no bullets left. The gun didn't fire between shots so it's chamber should have been open in the first place.

Other: In the scene where Dr. Isaacs is in the teleconference with the Umbrella Board of Directors, there are several glasses of water sitting in front of each individual hologram. At the end of the teleconference, the holograms of the board members disappear, but none of the glasses follow suit. This tells us that the glasses of water were physically in the room with Dr. Isaacs, for absolutely no reason, given all the other attendees weren't really there.

Visible crew/equipment: After the first Alice clone dies in the movie and Dr. Issacs takes off his helmet, look at the scientist to HIS right(our left) you can see the boom operator in his reflective mask along with the camera operator.

Factual error: In Las Vegas Paris and The Vencian are not next to each other. They farther part then that. Next to Paris is Ballys.

Plot hole: In the scene where Alice breaks into the Umbrella compound she drives the vehicle through the truck wreckage and into the fence, knocking the fence down. However, she then has time to ferry everyone from the vehicle into the chopper, stand back and watch them fly away, then walk cautiously into the shanty shack and wait for the elevator to arrive. Meanwhile not a single zombie has chased her into the now breached compound. The zombies might be dumb, but if they see food they head straight to it until an obstacle stops them and the explosion would have only killed the ones in the immediate area. The ones behind the dead zombies would have charged at them as soon as the fire died down.

Continuity: In the scene where Dr. Isaacs escapes from Alice in a AS350B Eurocopter, we see that the helicopter's landing gear has an additional iron bar that has been changed from one scene to another. When the helicopter lands in the desert, the bar is missing. So Dr. Isaacs flew away in one helicopter and landed in another.